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Treponema pallidum / Syphilis. Louisa Lu Cytoplasmic inclusions on Giemsa stain. 10% Gram negative, oxidase positive, comma shaped bacteria growing at 42 Various special stains have been applied. The specific immunohistochemical stain for Treponema pallidum is highly specific and sensitive and reveal the organisms to be delicate and spiral shaped (figure 1, inset). Silver impregnation techniques such as Warthin Starry highlight the organisms. Biochemical Structure  Treponema is composed of approximately 70 % proteins, 20 % lipids, and 5 % carbohydrates.  This lipid content is relatively high.

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Owing to its very thin structure, it is not easily seen on Gram stain, but can be visualized using dark-field microscopy. This spirochete contains endoflagella, which allow for a characteristic corkscrew movement. The bacteria are able to avoid immune recognition and phagocytosis by forming The cells stain gram-negative. The outer membrane of Treponema cells is similar to the outer membrane of gram-negative bacteria cells. The content includes lipids, proteins, and carbohydrates.

Due to its coat of lipids, it is hard to stain the organism, even though it would be gram-negative.

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We therefore evaluated the Treponema pallidum immunostain, which cross-reacts with Brachyspira species, as a diagnostic tool for IS. Treponema is a gram-negative, microaerophilic spirochete. Owing to its very thin structure, it is not easily seen on Gram stain, but can be visualized using dark-field microscopy.

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The content includes lipids, proteins, and carbohydrates. The lipids are mainly made up of phospholipids and glycolipids, while the cell wall contains muramic acid, glucosamine, and ornithine. Treponema pallidum är en tunn spiroket (5-15 µm lång och 0,15 µm tjock) som orsakar syfilis, yaws och bejel beroende på underart. [1] [2] Syfilis sexuellt överförbar, men kan även smitta foster om mamman är en bärare tidigt under graviditeten. Treponema pallidum can be considered a gram-negative bacterium although its cell envelope differs from other gram-negative bacteria. T. pallidum causes syphilis, a sexually transmitted disease that affects the skin and mucous membrane of the external genitalia, and also sometimes the mouth.

Treponema pallidum can be considered a gram-negative bacterium although its cell envelope differs from other gram-negative bacteria. T. pallidum causes syphilis, a sexually transmitted disease that affects the skin and mucous membrane of the external genitalia, and also sometimes the mouth.
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[9] 1. Evolution of Treponema Pallidum (Tr. Pallidum):.

Cultivation of Treponema Pallidum (Tr. Treponema pallidum is a Gram-nonreactive spirochete which causes treponemal diseases such as syphilis. Under the light microscope, this organism can only be seen using dark field microscopy. Due to its coat of lipids, it is hard to stain the organism, even though it would be gram-negative.
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In this test, T. pallidum antigen is coated on to the surface of red blood cells, and specific antibody in test sera causes haemagglutination. taining to the staining of Treponemrba pallidum was presented by Campbell and Rosahn (2), and recently DeLamater and others (3) have described a new modification of the Fontana staining procedure. Campbell and Rosahn classify all previously recommended procedures for staining T. pallidum into two groups. In the first group, the spirochetes are impregnated Gram Stain: Gram-negative, difficult to stain. Morphology: Helical rods.

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Description and significance Treponema pallidum is a Gram-negative bacteria which is spiral in shape. It is an obligate internal parasite which causes syphilis, a chronic human disease. Syphilis is a sexually transmitted disease but transmission can also occur between mother and child in utero; this is called congenital syphilis. T. pallidum can be localized on formalin fixed paraffin embedded tissue; the antibody has a rabbit purified IgG fraction (J Cutan Pathol 2004;31:595) Essential features Immunohistochemical stain for T. pallidum is more sensitive (71% sensitive) than silver stains - Warthin-Starry or Steiner (41% sensitive) ( J Cutan Pathol 2004;31:595 ) T. pallidum generally stains poorly with many dyes but can be visualized using silver impregnation techniques. Like other Gram-negative bacteria, T. pallidum has an outer membrane, an inner membrane, and a thin cell wall consisting of peptidoglycan. The outer membrane is unusual, however, in that it lacks lipopolysaccharide, thus rendering it more susceptible to damage resulting from physical disruption or detergent use during handling. Gram Stain: Gram-negative, difficult to stain.

Treponema pallidum can be visualized via standard light microscopy with a modified Gram's stain Treponema pallidum is a motile spirochete bacterium that is approximately 6 to 20 micrometers in length Treponema pallidum is an atypical gram-negative bacterium that lacks lipopolysaccharide and is approximately 5 by 2 micrometers in size An example of is Treponema pallidum, the causative organism of syphilis. Gram-Staining. The second way of classifying bacteria is according to Gram-staining.